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Nonreceptor (peptide) phosphorylation. Michaelis-Menten plots (Vi/[E]) are shown for peptide C phosphorylation either by bGRK1535-H6-WT (closed squares), by the R191A mutant (open squares), or by the ΔN19 truncation (open circles). Both the R191A and ΔN19 variants are compromised in their ability to phosphorylate peptide C. Kinetic parameters are listed in Table 3.
