FIGURE 3.

Identification of a PtdOH binding domain in TGD2C. A, primary structure of TGD2, indicating a predicted transit peptide (TP), transmembrane domain (TMD), and a conservative MCE domain. B and C, series of deletion and truncation mutants were generated for TGD2C and fused to the C terminus of the DR open reading frame. The black oval represents the DR carrier protein; gray bars represent deletions. Liposome association assays were performed to assess binding of various mutants to PtdCho and PtdOH/PtdCho mixtures or PtdOH liposomes. Fluorograms of Western blots are shown. PtdOH-specific binding data are summarized on the right based on visually examined signal intensity (+++++, ++++, +++, ++, and +, decreasing signal intensity; −, no binding).