FIGURE 5.

Functional analysis of basic residue mutations of MDA5 on the basic surface. A, MDA5^−/− mouse embryo fibroblasts were transfected with the reporter gene, p-125Luc, and pRL-tk, together with the expression vector for MDA5 and mutants. Cells were stimulated by transfection with poly(I:C) and subjected to a dual-luciferase assay. The values are the means ± S.D. from triplicate experiments. The relative luciferase activity was calculated by considering the luciferase activity from cells transfected with empty vector (BOS) as 1.0. The cell lysates were analyzed for expression of MDA5 and mutants by Immunoblotting (B).