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. 2009 Apr 20;284(26):17465–17474. doi: 10.1074/jbc.M109.007179

FIGURE 7.

FIGURE 7.

Effect of the mutations of LGP2 on RNA recognition. Full-length wild-type LGP2 and mutants on the RNA binding loop were produced in 293T cells and purified by anti FLAG antibody. A, silver staining of the purified recombinant LGP2. The recombinant LGP2 were subjected to EMSA using (B) 32P-labeled dsRNA (25/25c) or (C) 5′ppp-ssRNA (C; 5′pppGG25) as probe. The arrows indicate the RNA-LGP2 complex.