FIGURE 2.
hFFA agonists acutely promote phosphorylation of the ERK1/2 MAP kinases via activation of Gαq/Gα11. Cells were induced with doxycycline to induce expression of hFFA1-eYFP, as in Fig. 1, and then treated with or without inhibitors to block activation of Gαq/Gα11 (20 nm YM-254890 for 40 min, YM), Gαi/Gαo (25 ng/ml for 16 h; pertussis toxin, PTox), FFA1 (10 μm GW1100 (GW) for 40 min), or PPARγ (1 μm T0070907 or GW9662 for 40 min). Cells were then stimulated with lauric acid (100 μm, 10 min), rosiglitazone (100 μm, 30 min), or GW9508X (10 μm, 10 min). Samples were processed to detect phosphorylated (upper panels) and total (lower panels) levels of the ERK1/2 MAP kinases (n = 3). IB, immunoblot.