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Orthosteric binding site mutants of FFA1 display different responses to rosiglitazone when compared with lauric acid or GW9508X. Cells induced to express wild type (WT) hFFA1-eYFP or each of the individual mutants were then used to monitor potential activation of the ERK1/2 MAP kinases using the SureFire AlphaScreen assay. Response to the ligands was measured at 15 min of stimulation (n = 3). The error bars indicate S.E.
