Phenotypic variation in expression of sea urchin SRCR genes types 1 and
7. Blots of coelomocyte RNA and the corresponding genome blots of 11
animals were repeatedly hybridized with specific SpSRCR1 and SpSRCR7
probes. (Top) SpSRCR1: SRCR1-ECM, ECM domain probe;
(Middle) SpSRCR7: SRCR7-EC, an SRCR domain probe;
(Bottom) SpSRCR7.1: SRCR7-IC1, cytoplasmic 1 domain
probe (the segments corresponding to these probes are underlined in
Fig. 1). In the RNA blot, there are 10 μg of total RNA per lane, and
in the DNA blot, there are 5 μg of sperm DNA per lane, digested with
EcoRI (E) or HindIII (H). Numbers to the
left of the blots indicate length (kb).