Figure 4.

Clustered genes encoded in type 1 SRCR gene locus. An SRCR 1 clone of sea urchin genomic DNA in BAC plasmid vector (133-kb insert) was digested with NotI (N), EcoRI (E), or SalI (S), and the blot was probed repeatedly with specific segments of SpSRCR1: exon 1, the SRCR1 repeat element, the ECM domain and vWF domain. A cluster consisting of at least three closely related type 1 SRCR genes was detected by all four probes. Also shown is the ethidum bromide (EtBr) image of the pulsed-field gel corresponding to the blotted DNA; the BAC vector migrates at 8.9 kb in the N lane.