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. 2009 Apr 29;284(26):17648–17656. doi: 10.1074/jbc.M902005200

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© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Effect of GFP-α6-tubulin pseudophosphorylated mutants on motility of MCF-10A cells. A, lysates from MCF-10A transfectants (25 μg/lane) expressing recombinant WT GFP-α6-tubulin, pseudophosphorylated GFP mutants (S165D, S158D, S241D, or T337D), or VC were analyzed by Western blot using α-tubulin monoclonal antibody to detect the GFP fusion proteins (75 kDa) and endogenous α-tubulin (50 kDa). Note that the phosphorylation-resistant mutant S165N was included for comparison. The results were observed in three independent experiments. B, measurement of motility was carried out with MCF-10A cells that had been transfected with single site-pseudophosphorylated mutants of GFP-α6-tubulin, WT PKCα, or the vector control. Each value is the average of triplicate measurements ± S.D. The results are representative of three independent experiments.