FIGURE 4.

Specificity of palmitoylation by Pfa3 and Akr1. Increasing amounts of myr-Vac8-myc-6xHIS (A), myr-Meh1-myc-6xHIS (B), myr-Vac8[SH4]-GFP-6xHIS (C), or myr-Meh1[SH4]-GFP-6xHIS (D) were incubated with [³H]palmitoyl-CoA and either no enzyme (●), 10 nm Pfa3-FLAG (▲), or 10 nm Akr1-FLAG (■). Reactions were analyzed by fluorography and quantitated by densitometry with exposure lengths ranging from 8 h to 2 days. Data are the average of three independent experiments. The error bars represent the standard deviation. Representative films are shown in supplemental Fig. S3A, and 100% values are shown in supplemental Table 1.