Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 May 5;284(26):17720–17730. doi: 10.1074/jbc.M109.005447

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2009 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 5. — Palmitoylation of Meh1[SH4]-Vac8. A, membranes expressing Akr1-FLAG, FLAG-Erf2/GST-Erf4, Pfa3-FLAG, Pfa4-FLAG, Pfa5-FLAG, or empty vector (pESC) were incubated with [³H]palmitoyl-CoA and 1 μm myr-Meh1[SH4]-Vac8-myc-6xHIS. Reactions were analyzed by fluorography with an exposure length of 2 days. B, increasing amounts of myr-Meh1[SH4]-Vac8-myc-6xHIS was incubated with [³H]palmitoyl-CoA and either no enzyme (●), 10 nm Pfa3-FLAG (▲), or 10 nm Akr1-FLAG (■). Reactions were analyzed by fluorography and quantitated by densitometry with an exposure length of 2 days. Data are the average of three independent experiments. The error bars represent the standard deviation. Representative films are shown in supplemental figure S3A, and 100% values are shown in supplemental Table 1.