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Phenotypic analysis of DsbA chimeras
All dsbA genes were cloned into the low copy vector pHSG576 and assessed for their ability to restore E. coli dsbA mutant strain JCB571 resistance to DTT and motility.
| dsbA | DTT resistance | Motility |
|---|---|---|
| JCB570 (wild type) | Yes | Yes |
| JCB571 (dsbA−) | No | No |
| JCB571 transformed with | ||
| EcDsbA | Yes | Yes |
| EcTDNMDsbA1α | Yes | No |
| EcTDNMDsbA1α (QLRGV) | Yes | Yes |
| EcTDNMDsbA2α | Yes | Yes |
| EcTDNMDsbA1α (QLRGV) | Yes | Yes |
