TABLE 3.
Sequence conservation in DsbA substrates
Forty-five sequences of confirmed substrates of DsbA were compared to assess sequence conservation around the cysteine residue. Analysis of the sequences revealed that there was no conservation beyond the cysteine residue itself, as determined using Conscore.
| Conservative mutations | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Hydrophobic, small (AVLI) | 17 | 9 | 13 | 8 | 13 | 0 | 15 | 15 | 11 | 5 | 7 |
| Hydrophobic, aromatic (FYW) | 2 | 8 | 3 | 7 | 3 | 0 | 3 | 4 | 4 | 5 | 2 |
| Acidic (DE) | 6 | 5 | 6 | 5 | 7 | 0 | 5 | 4 | 2 | 7 | 9 |
| Basic (RHK) | 7 | 4 | 5 | 6 | 1 | 0 | 5 | 6 | 6 | 8 | 4 |
| Neutral polar (SMTCNQ) | 10 | 15 | 9 | 14 | 18 | 45 | 14 | 9 | 13 | 18 | 15 |
| Glycine | 2 | 3 | 6 | 4 | 2 | 0 | 2 | 1 | 5 | 1 | 2 |
| Proline | 1 | 1 | 3 | 1 | 1 | 0 | 1 | 6 | 4 | 1 | 5 |
| Percentage of conserved mutations | 40.5 | 36.6 | 36.1 | 35.0 | 42.9 | 100.0 | 35.7 | 39.5 | 36.1 | 41.9 | 40.5 |