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Determination of [3H]MTX influx in R1-11 cells transiently transfected with WT-pcft and mutant constructs in which His residues were substituted with Ala. Influx of [3H]MTX (0.5 μm) was assessed at pH 5.5 and 37 °C over 1 min. Data are the mean ± S.E. from three independent experiments.
