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A, [3H]folic acid influx kinetics in R1-11 cells transiently transfected with WT-pcft or the H247A mutant at pH 5.5. B, [3H]folic acid influx kinetics in R1-11 cells transiently transfected with WT-pcft or the H281A mutant at pH 5.5. Data are the mean ± S.E. from three independent experiments. Insets: concentrations of substrates were adjusted to bracket the influx Kt. Influx was determined over 1 min.
