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A, inhibition of 0. 5 μm [3H]MTX influx by 2 μm 5-methyl-THF, 5-formyTHF, or folic acid in R1-11 cells transiently transfect with WT-pcft, or His mutants. Data are the mean ± S.E. from three independent experiments. B, the data in A presented as percentage of the control rate for each construct in the absence of inhibitor. Data are the mean ± S.E. from three independent experiments. Influx was determined at pH 5.5 over 1 min.
