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The pH dependence of MTX influx for WT PCFT and the H281A mutant. A, pH dependence of [3H]MTX influx in cells transfected with WT-pcft or the H281A mutant. B, [3H]MTX influx over a pH range of 6.0–4.5. Data are plotted as -fold change in [3H]MTX influx at pH 6.0, which is set to 1. Data are the mean ± S.E. from three independent experiments. Influx was assessed over 1 min; the [3H]MTX concentration was 0.5 μm.
