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. 2009 Apr 21;284(26):17868–17876. doi: 10.1074/jbc.M900368200

FIGURE 5.

FIGURE 5.

Effects of NF-κB and/or AP-1 inhibition on IL-32 mRNA expression. A–C, PANC-1 cells were infected with an adenovirus expressing the IκBΔN or DN-c-Jun, and at 48 h after infection, cells were stimulated with IL-1β (10 ng/ml), TNF-α (100 ng/ml), or IFN-γ (100 ng/ml) for 12 h. IL-32 mRNA expression was determined by real time PCR. Adenovirus expressing LacZ was used as a negative control. The data were expressed by IL-32 mRNA expression relative to β-actin mRNA expression (mean ± S.D. from four different experiments). **, p < 0.01. D and E, electrophoretic gel mobility shift assays for NF-κB and/or AP-1 DNA binding activities. PANC-1 cells were incubated with medium alone, IL-1β (10 ng/ml), TNF-α (100 ng/ml), or IFN-γ (100 ng/ml) with or without LY294002 (25 μm) for 1.5 h, and then nuclear extracts were prepared.