FIGURE 1.

15-Lipoxygenase-1 and -2 and h 12-LOX protein expression in ARPE-19 silenced cells. Both the stable cell line expressing shRNA targeting 15-LOX-1 as well as cells expressing a nonspecific scrambled targeting sequence (NS) were analyzed. A, D, and K show representative immunoblot of at least three experiments along the different cell passages. 15-LOX-1, h12-LOX, and 15-LOX-2 expressed in the knockdown cells were plotted as ratios between enzyme and the housekeeping control (glyceraldehyde-3-phosphate dehydrogenase (GAPDH)) in relation with the control expressing nonspecific shRNA. These two cell types were immunostained against 15-LOX-1 in green (B and E) and actin in red (C and F). 15-LOX-2 signal (red) and actin (green) are shown in G–J. The nuclei were stained with 4′,6-diamidino-2-phenylindole, blue (B, C, and E–J).