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. Author manuscript; available in PMC: 2009 Aug 1.
Published in final edited form as: Nat Struct Mol Biol. 2009 Jan 25;16(2):176–182. doi: 10.1038/nsmb.1546

Figure 2. Suppressor U1 snRNAs in the shifted register can rescue splicing.

Figure 2

a, Schematic of the single mutations introduced at the atypical 5' ss in the SMN1/2 context. These mutations substitute a non-consensus nucleotide by a consensus nucleotide. b, Base-pairing of the mutant 5' ss with the corresponding suppressor U1 snRNA. As an example, we show the base-pairing of the +5G mutant 5' ss with the suppressor U1 snRNA carrying the corresponding compensatory mutation (C5) in the shifted register. The mutant nucleotide at the 5' end of U1 in each case is shown in red. See Supplementary Fig. 2 online for the base-pairing of all mutant 5' ss with their respective suppressor U1s. c, RT-PCR analysis of the SMN1/2 minigenes carrying the wild-type (lane 1) or mutant atypical 5' ss (lanes 2–13). The 5' ss mutation is indicated at the top, without (−) or with (+) the corresponding suppressor U1 snRNA. The mRNA products are schematically indicated on the left of each panel. The fastest migrating band in SMN1 corresponds to an mRNA that skipped exon 7 and used a cryptic 5' ss 50 nucleotides upstream of the exon 6 5' ss. The percentage and Standard Deviation (SD) of exon 7 inclusion is indicated below each autoradiogram.