Skip to main content
. Author manuscript; available in PMC: 2009 Jul 31.
Published in final edited form as: Cell Host Microbe. 2007 Sep 13;2(3):181–192. doi: 10.1016/j.chom.2007.07.003

Figure 4. Rab34 Localizes to Tight Junctions and Mediates Occludin Internalization.

Figure 4

(A) Monolayers were exposed to CVB (100 PFU/cell) for 30 min at 37°C and then fixed and stained for occludin (red) and Rab34 (green).

(B) Cells expressing wild-type EGFP-Rab34 were fixed and stained for ZO-1 (red).

(C) Colocalization of EGFP-Rab34 and DsRed-caveolin-1. Insert, 33 zoomed image.

(D) Cells expressing wild-type (WT) or constitutively active (Q111L) EGFP-Rab34 were fixed and stained for occludin (red) and ZO-1 (blue).

(E) Quantification of occludin internalization in cells transfected with a GFP control plasmid (CON), or wild-type (WT), constitutively active (Q111L) EGFP-Rab34, dominant-negative (T66N) Rab34. Cells were either exposed to no virus (−CVB) or exposed to CVB (+CVB) for 90 min at 37°C. Data are representative of at least three independent experiments performed three times. Data in (E) are shown as mean ± standard deviation. *p < 0.05.