Skip to main content
. Author manuscript; available in PMC: 2009 Jul 31.
Published in final edited form as: Cell Host Microbe. 2007 Sep 13;2(3):181–192. doi: 10.1016/j.chom.2007.07.003

Figure 5. Rab34 Is Required for CVB Entry and Infection.

Figure 5

(A) Monolayers expressing dominant-negative (T66N), constitutively active (Q111L), or wild-type forms of EGFP-Rab34 were exposed to CVB (1 PFU/cell), and stained for VP1 at 7 hr p.i. Data are presented as the percentage of transfected cells expressing VP1.

(B) Western blot analysis of Caco-2 cells 48 hr after transfection with the indicated siRNAs. All blots were stripped and reprobed with GAPDH pAb to control for equal protein loading. Densitometry was performed with Image J to determine efficacy.

(C) Cells transfected with control (CON), Rab34, Rab7, or Rab13 siRNAs were exposed to CVB (1 PFU/cell) and stained for VP1 at 7 hr p.i.

(D) Quantification of the effects of CON, Rab34, Rab7, and Rab13 siRNAs on CVB and occludin internalization. Data are shown as percentage of cells containing internalized CVB and occludin.

(E) Cells transfected with control (CON), Rab34, or Rab13 siRNAs were exposed to CVB (100 PFU/cell) and stained for VP1 at 90 min p.i. Data are representative of at least three independent experiments performed three times. Data in (A), (C), and (D) are shown as mean ± standard deviation. *p < 0.05.