Figure 5.

Hog1 can induce an S phase delay independent of S-CDK activity. Cells of the S-CDK bypass system (Zegerman and Diffley, 2007) capable of initiating replication without Cdc28-Clb5,6 activity were synchronized in α-factor for 3 h (α-fac) in either glucose (left) or in galactose, in which Sic1ΔNT is expressed and Cdc28-Clb5/6 activity is inhibited. Cells were liberated of α-factor into fresh YPD (control). Half of the culture was exposed to 0.4 M NaCl 20 min after release from α-factor (NaCl).