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. Author manuscript; available in PMC: 2010 Apr 17.
Published in final edited form as: Immunity. 2009 Apr 17;30(4):521–532. doi: 10.1016/j.immuni.2009.03.007

Figure 2. DKF-2 Deficient Animals Are Hypersensitive to Pathogenic Bacteria; Elevated DKF-2-GFP Expression Confers Pathogen Resistance.

Figure 2

A shows survival curves for WT (N2), dkf-2(pr3) null, transgenic (dkf-2::DKF-2-GFP and dkf-2(pr3);dkf-2::DKF-2-GFP) C. elegans fed with PA14. Assays were replicated 4 times with similar results; typical data are shown. B, proteins (30 μg/lane) from the indicated animals were separated by denaturing electrophoresis and transferred to a PVDF membrane. The blot was probed with anti-DKF-2 IgGs. C shows survival curves for animals fed with E. faecalis OG1RF. Assays were replicated 3 times with similar results. D, WT or transgenic animals were fed with OP50, OP50 HK or PA14 as indicated. Animals were disrupted in a French press, using buffer containing 1% Triton-X100. DKF-2 and DKF2-GFP were immunoprecipitated from samples containing 0.5 mg protein (from equal numbers of animals) by anti-DKF-2 IgGs. Precipitated proteins were assayed for kinase activity.

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