Figure 2.

Treatment with TP compounds at low concentrations is not toxic for primary neuronal cultures. A. Survival of primary striatal neurons from control mice treated with different concentrations of CP2. Days in culture (DIC) and CP2 concentrations are indicated. Images were acquired using LSM 510 confocal microscope with 63 × or 100 × oil DIC lenses (1.4 na). Scale bars, 20 um. B. Lethal dose at which 50% of the neurons died (LD₅₀) for all three TP compounds was estimated at day 6 after plating using LIVE/DEAD assay.