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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Nat Methods. 2009 Jul 20;6(8):593–595. doi: 10.1038/nmeth.1354

Figure 1. Virtual Terminator nucleotide base-by-base incorporation in a G5 homopolymer.

Figure 1

The substrate used for testing homopolymer sequencing is shown along with successive cycles of addition of compound 22 in a solution phase reaction. Removal of the inhibitor-dye was accomplished by cleavage of the disulfide using TCEP, a reducing agent, followed by treatment with iodoacetamide to cap the free thiol. After each cycle, an aliquot of the reaction is run on an ABI3730 sequencing machine to achieve single base resolution of DNA. Length markers are shown in orange and the DNA being synthesized is shown in blue.