Table 2.
Fold change in gene expression of septic to normal sera treated cardiac myocytes using the 1.7K human cDNA array
| Genbank Accession ID | Gene Symbol | Description | Fold Change |
|---|---|---|---|
| Apoptosis | |||
| AA136799 | GADD45A | growth arrest and DNA-damage-inducible, alpha | 0.28 |
| N34233 | MAGEH1 | melanoma antigen family H, 1 | 0.33 |
| BG620906 | CSE1L | CSE1 chromosome segregation 1-like (yeast) | 0.46 |
| T78285 | CRADD | CASP2 and RIPK1 domain containing adaptor with death domain | 0.51 |
| N91060 | VEGF | vascular endothelial growth factor | 0.53 |
| AA011445 | CASP3 | caspase 3, apoptosis-related cysteine peptidase | 0.59 |
| W92108 | DHCR24 | 24-dehydrocholesterol reductase | 0.60 |
| H79188 | ERCC2 | excision repair cross-complementing rodent repair deficiency, complementation group 2 (xeroderma pigmentosum D) | 0.65 |
| BI824641 | BTK | Bruton agammaglobulinemia tyrosine kinase | 0.70 |
| BG387747 | API5 | apoptosis inhibitor 5 | 2.21 |
| Catalysts | |||
| W31103 | BRD2 | bromodomain containing 2 | 0.53 |
| AA039228 | FNTB | farnesyltransferase, CAAX box, beta | 0.54 |
| H11807 | CTPS | CTPsynthase | 0.57 |
| H62727 | CRYM | crystallin, mu | 0.58 |
| N77157 | PTPN12 | protein tyrosine phosphatase, non-receptor type 12 | 0.61 |
| AA031513 | MMP7 | matrix metallopeptidase 7 (matrilysin, uterine) | 0.64 |
| BE738657 | PBEF | pre-B-cell colony enhancing factor 1 | 0.65 |
| N50000 | MAT1A | methionine adenosyltransferase I, alpha | 0.65 |
| W92066 | RFC4 | replication factor C (activator 1) 4, 37kDa | 0.66 |
| Cell adhesion | |||
| H29191 | APC | adenomatosis polyposis coli | 0.08 |
| BI496175 | ALCAM | activated leukocyte cell adhesion molecule | 0.47 |
| H16046 | ITGA6 | integrin, alpha 6 | 0.52 |
| H30141 | SELP | selectin P (granule membrane protein 140kDa, antigen CD62) | 0.60 |
| T80274 | CD34 | CD34 antigen | 0.64 |
| W01300 | ITGB3 | integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61) | 0.66 |
| Cell cycle | |||
| T65624 | DUSP6 | dual specificity phosphatase 6 | 0.16 |
| H71112 | MCM2 | MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae) | 0.46 |
| H14471 | MCM6 | MCM6 minichromosome maintenance deficient 6 (MIS5 homolog, S. pombe) (S. cerevisiae) | 0.46 |
| BG424365 | SES2 | sestrin 2 (SESN2) | 0.52 |
| H04421 | DUSP1 | dual specificity phosphatase 1 | 0.55 |
| AA001329 | CCNA2 | cyclin A2 | 0.62 |
| BG403841 | NHP2L1 | NHP2 non-histone chromosome protein 2-like 1 (S. cerevisiae) | 0.62 |
| R14363 | HDAC5 | histone deacetylase 5 | 1.88 |
| Cell proliferation | |||
| AA128253 | PMP22 | peripheral myelin protein 22 | 0.30 |
| AI819719 | PDGFRA | platelet-derived growth factor receptor, alpha polypeptide | 0.30 |
| AA114957 | MXI1 | MAX interactor 1 | 0.45 |
| W95000 | CDC25C | cell division cycle 25C | 0.47 |
| W47595 | TGFB2 | transforming growth factor, beta 2 | 0.48 |
| AA028183 | TGFBR2 | transforming growth factor, beta receptor II (70/80kDa) | 0.53 |
| H29950 | TOB1 | transducer of ERBB2, 1 | 0.58 |
| BG565696 | PRDX1 | peroxiredoxin 1 | 0.60 |
| AA055757 | CSRP2 | cysteine and glycine-rich protein 2 | 0.60 |
| AA037107 | TGFA | transforming growth factor, alpha | 0.60 |
| AA044049 | GNRH1 | gonadotropin-releasing hormone 1 | 0.61 |
| H12419 | USP8 | ubiquitin specific peptidase 8 | 0.63 |
| W17355 | DTR | heparin-binding EGF-like growth factor (HBEGF) | 0.63 |
| AA007492 | RBBP4 | retinoblastoma binding protein 4 | 0.64 |
| W19744 | CDK6 | Cyclin-dependent kinase 6 (CDK6) | 0.67 |
| W78793 | EPS8 | epidermal growth factor receptor pathway substrate 8 | 0.69 |
| Cellular component organization and intracellular transport | |||
| AA056151 | SEC24C | SEC24 related gene family, member C (S. cerevisiae) | 0.27 |
| BG753663 | TUBB5 | tubulin, beta 5 | 0.52 |
| H59171 | SEC23B | Sec23 homolog B (S. cerevisiae) | 0.52 |
| W01720 | SRPR | signal recognition particle receptor (‘docking protein’) | 0.57 |
| W92260 | RAB11A | RAB11A, member RAS oncogene family | 0.58 |
| W33064 | TUBA1 | tubulin, alpha 1 (testis specific) | 0.58 |
| BG169044 | ARL6IP | ADP-ribosylation factor-like 6 interacting protein | 0.58 |
| W48577 | GARS | glycyl-tRNAsynthetase | 0.60 |
| R20063 | TPR | translocated promoter region (to activated MET oncogene) | 0.62 |
| W16514 | RHO6 | Rho family GTPase 1 (RND1) | 0.63 |
| AA053988 | ARF1 | ADP-ribosylation factor 1 | 0.64 |
| N31521 | BPAG1 | dystonin (DST) | 0.64 |
| BG770889 | Rab11-FIP2 | RAB11 family interacting protein 2 (class I) | 0.64 |
| H28534 | AQP1 | aquaporin 1 (channel-forming integral protein, 28kDa) | 0.64 |
| N34169 | NF2 | neurofibromin 2 (bilateral acoustic neuroma) | 0.65 |
| Developmental processes | |||
| BG740719 | DDX5 | DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 | 0.31 |
| AA037738 | HGF | hepatocyte growth factor (hepapoietin A; scatter factor) | 0.38 |
| H09372 | PPP2CA | protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform | 0.41 |
| T75267 | FRAP1 | FK506 binding protein 12-rapamycin associated protein 1 | 0.43 |
| N40420 | CCND1 | cyclin D1 | 0.51 |
| W48569 | BDNF | brain-derived neurotrophic factor | 0.52 |
| H52752 | HSPA9B | heat shock 70kDa protein 9B (mortalin-2) | 0.55 |
| W49766 | WNT5A | wingless-type MMTV integration site family, member 5A | 0.55 |
| W01469 | ETS2 | v-ets erythroblastosis virus E26 oncogene homolog 2 (avian) | 0.61 |
| N25434 | MSX2 | msh homeo box homolog 2 (Drosophila) | 0.62 |
| W38673 | ID2 | inhibitor of DNA binding 2, dominant negative helix-loop-helix protein | 0.63 |
| BI198063 | IGFBP2 | insulin-like growth factor binding protein 2, 36kDa | 0.67 |
| DNA Repair | |||
| AA134026 | UBE2A | ubiquitin-conjugating enzyme E2A (RAD6 homolog) | 0.41 |
| T78280 | HAT1 | histone acetyltransferase 1 | 0.43 |
| AA028978 | ERCC5 | excision repair cross-complementing rodent repair deficiency, complementation group 5 (xeroderma pigmentosum, complementation group G (Cockayne syndrome)) | 0.56 |
| H30857 | MLH1 | mutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) | 0.63 |
| Integral to plasma memebrane | |||
| H29706 | GPC5 | glypican 5 | 0.37 |
| W05301 | CD1E | CD1E antigen, e polypeptide | 0.55 |
| BF212425 | RAI3 | G protein-coupled receptor, family C, group 5, member A (GPRC5A) | 0.59 |
| Metabolism | |||
| BG762226 | PCSK2 | proprotein convertase subtilisin/kexin type 2 | 0.40 |
| N63511 | DPYD | dihydropyrimidine dehydrogenase | 0.55 |
| T99689 | MTR | 5-methyltetrahydrofolate-homocysteine methyltransferase | 0.55 |
| H15303 | PCCB | propionyl Coenzyme A carboxylase, beta polypeptide | 0.57 |
| H19387 | ME2 | malic enzyme 2, NAD(+)-dependent, mitochondrial | 0.57 |
| BI193156 | SDF2 | stromal cell-derived factor 2 | 0.58 |
| AA029397 | PSMA6 | proteasome (prosome, macropain) subunit, alpha type, 6 | 0.58 |
| BE254368 | FDPS | farnesyl diphosphate synthase (farnesyl pyrophosphate synthetase, dimethylallyltranstransferase, geranyltranstransferase) | 0.59 |
| AA029803 | HDLBP | high density lipoprotein binding protein (vigilin) | 0.59 |
| BI160119 | RPL27A | ribosomal protein L27a | 0.59 |
| R98094 | BPGM | 2,3-bisphosphoglycerate mutase | 0.60 |
| R52371 | SLC35A1 | solute carrier family 35 (CMP-sialic acid transporter), member A1 | 0.61 |
| H30710 | BAT1 | HLA-B associated transcript 1 | 0.63 |
| R01732 | AMPD3 | adenosine monophosphate deaminase (isoform E) | 0.63 |
| W68191 | CLU | clusterin (complement lysis inhibitor, SP-40,40, sulfated glycoprotein 2, testosterone-repressed prostate message 2, apolipoprotein J) | 0.63 |
| N31054 | MPV17 | MpV17 transgene, murine homolog, glomerulosclerosis | 0.64 |
| W94208 | FDFT1 | farnesyl-diphosphate farnesyltransferase 1 | 0.64 |
| W92014 | ADH6 | alcohol dehydrogenase 6 (class V) | 0.64 |
| W52843 | ALDH3B1 | aldehyde dehydrogenase 3 family, member B1 | 0.65 |
| W31637 | LPL | lipoprotein lipase | 0.65 |
| H82585 | NT5E | 5′-nucleotidase, ecto (CD73) | 0.65 |
| W37213 | HEM1 | NCK-associated protein 1-like (NCKAP1L) | 0.66 |
| Metal binding | |||
| H93255 | MT1E | metallothionein 1E (functional) | 0.62 |
| R18562 | ATP7B | ATPase, Cu++ transporting, beta polypeptide | 0.62 |
| R99208 | MT1B | metallothionein 1B (functional) | 0.64 |
| Mitochondrial | |||
| W37672 | HK1 | hexokinase 1 | 0.60 |
| AA133258 | HADHB | hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit | 0.62 |
| W21228 | UQCRC2 | ubiquinol-cytochrome c reductase core protein II | 0.65 |
| N72504 | FECH | ferrochelatase (protoporphyria) | 2.4 |
| Nervous System(Development) | |||
| R15219 | EPHA7 | EPH receptor A7 (EPHA7) | 0.45 |
| H17696 | MBP | myelin basic protein | 0.46 |
| BI826246 | PLP1 | proteolipid protein 1 (Pelizaeus-Merzbacher disease, spastic paraplegia 2, uncomplicated) | 0.52 |
| Protein (folding/unfolding/proteolysis) | |||
| H18866 | CHRM3 | cholinergic receptor, muscarinic 3 | 0.31 |
| H03673 | KRT7 | keratin 7 | 0.48 |
| AA036988 | CCT3 | chaperonin containing TCP1, subunit 3 (gamma) | 0.53 |
| H62639 | HSPA8 | heat shock 70kDa protein 8 | 0.56 |
| W90284 | HERPUD1 | homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1 | 0.57 |
| H53489 | C4BPA | complement component 4 binding protein, alpha | 0.62 |
| R48054 | CCT5 | chaperonin containing TCP1, subunit 5 (epsilon) | 0.62 |
| AA031513 | MMP7 | matrix metallopeptidase 7 (matrilysin, uterine) | 0.64 |
| T74000 | BLMH | bleomycin hydrolase | 0.64 |
| Regulation of Translation (Initiators/Inhibitors) | |||
| H79856 | UK114 | (HRSP12) heat-responsive protein 12 | 0.29 |
| AA098888 | ETF1 | eukaryotic translation termination factor 1 | 0.58 |
| N79725 | MTIF2 | mitochondrial translational initiation factor 2 | 0.58 |
| BG178139 | WBSCR1 | Williams-Beuren syndrome chromosome region 1 | 0.63 |
| Signaling | |||
| T74308 | MAPK6 | mitogen-activated protein kinase 6 | 0.20 |
| AI267373 | RGS4 | Regulator of G-protein signalling 4 (RGS4) | 0.28 |
| H18866 | CHRM3 | cholinergic receptor, muscarinic 3 | 0.31 |
| AA055349 | AD0RA2B | adenosine A2b receptor | 0.46 |
| T97105 | TXNRD1 | thioredoxin reductase 1 | 0.52 |
| W79493 | PDE4B | phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila) | 0.56 |
| W56577 | VLDLR | very low density lipoprotein receptor | 0.56 |
| AA043909 | PTK2 | PTK2 protein tyrosine kinase 2 | 0.57 |
| T75436 | MAPK10 | mitogen-activated protein kinase 10 | 0.59 |
| N31391 | AKAP12 | A kinase (PRKA) anchor protein (gravin) 12 | 0.62 |
| AA001104 | RGS3 | regulator of G-protein signalling 3 | 0.62 |
| W35223 | ARHGDIB | Rho GDP dissociation inhibitor (GDI) beta | 0.62 |
| H15389 | NPY1R | neuropeptide Y receptor Y1 | 0.63 |
| W52156 | OXTR | oxytocin receptor | 0.63 |
| AA133259 | GEM | GTP binding protein overexpressed in skeletal muscle | 0.64 |
| AA056159 | RHO | rhodopsin (opsin 2, rod pigment) (retinitis pigmentosa 4, autosomal dominant) | 0.64 |
| R52300 | GABRA1 | gamma-aminobutyric acid (GABA) A receptor, alpha 1 | 0.65 |
| BF971558 | STMN1 | stathmin 1/oncoprotein 18 | 1.78 |
| Transcription factors and transcriptional regulators | |||
| W05657 | ELF1 | E74-like factor 1 (ets domain transcription factor) | 0.04 |
| AA044787 | CNOT8 | CCR4-NOT transcription complex, subunit 8 | 0.12 |
| AA127003 | HSZFP36 | ZFP-36 for a zinc finger protein | 0.18 |
| R59543 | NRIP1 | nuclear receptor interacting protein 1 | 0.23 |
| AA005274 | ZNF7 | zinc finger protein 7 (KOX 4, clone HF.16) | 0.24 |
| R35310 | MEIS2 | Meis1, myeloid ecotropic viral integration site 1 homolog 2 (mouse) | 0.24 |
| R20489 | ZNF16 | zinc finger protein 16 (KOX 9) | 0.25 |
| R80355 | BACH1 | BTB and CNC homology 1, basic leucine zipper transcription factor 1 | 0.31 |
| R13988 | EGR3 | early growth response 3 | 0.32 |
| T77424 | FHX | (FOXJ2) forkhead box J2 | 0.38 |
| T84358 | ZNF135 | zinc finger protein 135 (clone pHZ-17) | 0.41 |
| H14414 | ZNF195 | DKFZp434A2314 5′, mRNA sequence. | 0.42 |
| N31546 | IFI16 | interferon, gamma-inducible protein 16 | 0.43 |
| AA043380 | HOXD10 | homeo box D10 | 0.45 |
| N23578 | TCF12 | transcription factor 12 (HTF4, helix-loop-helix transcription factors 4) | 0.49 |
| W90498 | SSB | Sjogren syndrome antigen B (autoantigen La) | 0.52 |
| AA035361 | STAT1 | signal transducer and activator of transcription 1, 91kDa | 0.53 |
| N94714 | YY1 | YY1 transcription factor | 0.54 |
| H50875 | F0XA3 | forkhead box A3 | 0.56 |
| W32908 | FOXO1A | forkhead box O1A (rhabdomyosarcoma) | 0.61 |
| W38673 | ID2 | inhibitor of DNA binding 2, dominant negative helix-loop-helix protein | 0.63 |
| AA043457 | ZNF137 | zinc finger protein 137 (clone pHZ-30) | 0.64 |
| H27140 | ZNF35 | zinc finger protein 35 (clone HF.10) | 0.65 |
| N34004 | STAT6 | signal transducer and activator of transcription 6, interleukin-4 induced | 0.65 |
| R66676 | ETV5 | ets variant gene 5 (ets-related molecule) | 0.65 |
| W16724 | MLL | myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila) | 0.67 |
| Others | |||
| W03376 | NNMT | nicotinamide N-methyltransferase | 0.34 |
| T78107 | GRM5 | glutamate receptor, metabotropic 5 | 0.59 |
| W39552 | BMPR1A | bone morphogenetic protein receptor, type IA | 0.61 |
| BI457624 | PPIL2 | peptidylprolyl isomerase (cyclophilin)-like 2 | 0.62 |
| H92049 | LRBA | LPS-responsive vesicle trafficking, beach and anchor containing | 0.63 |
Table indicates differentially regulated genes from fetal cardiac myocytes treated 12 hours with sera from either healthy or septic individuals. Fold changes are the average ratios between the 4 septic and the 4 healthy individuals.