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. 2009 Jun 18;2(2):176–192.

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IJCEM Copyright © 2009

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Western analysis confirms the expression of NFAT-2 in cultured epidermal keratocytes and dermal fibroblasts. Cell lysates were prepared from cultured keratocytes and dermal fibroblasts, separated by SDS-PAGE and immunoblotted with anti-NFAT-2 (801) antibody. This experiment confirmed that antibodies used in immunostaining detects the appropriate molecular weight of two NFAT-2 isoforms (86 kDa and 116 kDa) (A and B). (A), lane 1, medium control (keratocytes) (donor 1); lane 2, medium control (keratocytes) (donor 2); lane 3, DMSO 4 h (keratocytes) (vehicle) (donor 2); lane 4, DMSO 4 h (vehicle) (keratocytes) (donor 1); lane 5, TPA/ionomycin 4 h (donor 2); lane 6, TPA/ionomycin 4 h (donor 1). (B), lane 1, medium control (fibroblasts) (donor 1); lane 2, medium control (fibroblasts) (donor 2); lane 3, DMSO 4 h (vehicle) (fibroblasts) (donor 2); lane 4, DMSO 4 h (fibroblasts) (vehicle) (donor 1).