Fig. 1.

The expression of APLP2 in human tumor cell lines varied greatly among the cell lines tested. Samples of the indicated cell lysates (made from equal numbers of cells from each cell line) were electrophoresed on a 4 → 20% Tris–glycine acrylamide gel, transferred to a Western blot, and probed with APLP2-specific antiserum for comparison of the total amount of APLP2 within the cells or with an antibody that recognizes β-actin. The results for APLP2 were normalized to the results for β-actin and the normalized results are displayed on the bar graph. The results shown are representative of the results obtained in three independent experiments