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. Author manuscript; available in PMC: 2010 Feb 1.
Published in final edited form as: Eur J Cell Biol. 2008 Oct 11;88(2):65–77. doi: 10.1016/j.ejcb.2008.08.004

Fig. 5.

Fig. 5

Expression analyses of Cx23 in stably transfected HeLa cells. Fluorescence microscopy of Cx23eGFP and Cx23-IRES-eGFP HeLa cells shows expression of the Cx23eGFP fusion protein in the plasma membranes of HeLa cells (A) and cytoplasmic eGFP in Cx23-IRES-eGFP HeLa cells (B). Bars: 20 μm. Northern blot analysis (C) of total RNA (D) from Cx23eGFP and Cx23-IRES-eGFP HeLa cells confirms expression of the corresponding mRNAs (1.8 kb for Cx23eGFP, 2.3 kb for Cx23-IRES-eGFP) in stably transfected HeLa cell lines. M: RNA ladder (Gibco-BRL).