Fig. 2.

Ca²⁺ mobilization in N18TG2 cells: cannabinoid agonists compared with bradykinin. N18TG2 cells loaded with 5 μM Fluo-4 AM were treated with 2-arachidonoylglycerol (2-AG), CP55940, WIN55212-2, or bradykinin (BK) at the indicated concentrations and fluorescence intensity evaluated using confocal microscopy. Data denote the percentage of total cells fluorescing above baseline over a 6-min experimental period and are presented as the mean ± SEM from N = 5–9 experiments