Fig. 2.

PPARβ pharmacological activation induces an increase of myonuclear density. a Quantification of myonuclei number in isolated DAPI-stained fibres (n = 160, four animals per group, 40 fibres per animal) harvested from tibialis anterior of 10-week-old C57Bl6 mice for different times of treatment with vehicle (Ct) or PPARβ agonist GW0742 (1 day (1d), 2 days (2d) and 4 days (4d)). *p < 0.001, different from vehicle-treated group (Ct); pound sign p < 0.01, different from 1 day treated animal group (1d). b Distribution of myofibres population counted in a using the same classification as in Fig. 1b. Percentage of each group, mean number of nuclei in each group (Mean) and standard deviation (s.d.) are reported for each period of treatment with GW0742