Figure 7.

The effect of inhibiting or depleting myosin-II on signaling in T cells. (a) Control or blebbistatin pretreated Jurkat T cells were added to a planer lipid bilayer containing Alexa-568 labeled TCR antibody and ICAM1 for 25 min. Cells were then fixed and stained with antibodies against Src^pY416, ZAP70^pY319 and LAT^pY191. Quantitative representation of relative protein phosphorylation is depicted on the right (n = 15 cells for each bar and error bars indicate standard deviation). (b) Primary human CD4⁺ cells treated with siRNA constructs either specific or non-specific for MYH9 gene were added to a planer lipid bilayer containing Alexa-568 labeled TCR antibody and ICAM-1 for 25 min. Cells were then fixed and stained with antibodies against Src^pY416, ZAP70^pY319 and LAT^pY191. Myosin IIA depleted cells were determined by the lack of central TCR clustering as demonstrated in Fig. 6b. Quantitative representation of relative protein phosphorylation is depicted on the right (n = 15 cells for each bar).