Figure 2.
(a) ROS levels in non-transgenic (open bars) and hIAPP transgenic (closed bars) islets following 48-hour culture in 5.5 mmol/L or 16.7 mmol/L glucose +/− the antioxidant, N-acetyl-L-cysteine (NAC; 5 mmol/L). Data are expressed relative to the fluorescence from 5.5 mmol/L glucose-cultured islets. NAC significantly decreased ROS levels in all islets, regardless of genotype or media glucose concentration. n=3; *p<0.01 vs non-transgenic.
Amyloid prevalence (b) and severity (c) in hIAPP transgenic islets following 48-hour culture in 16.7 mmol/L glucose +/− 5 mmol/L NAC. NAC treatment had no effect on amyloid prevalence or severity. n=5.