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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Diabetologia. 2009 Jan 16;52(4):626–635. doi: 10.1007/s00125-008-1255-x

Figure 7.

Figure 7

(a) ROS levels, (b) amyloid prevalence, (c) amyloid severity, (d) beta cell apoptosis and (e) insulin content in non-transgenic (open bars) and hIAPP transgenic (closed bars) islets following 144-hour culture in 16.7 mmol/L glucose +/− NAC or Congo red. ROS data are expressed relative to the fluorescence from 16.7 mmol/L glucose-cultured non-transgenic islets. Compared to non-transgenic islets, hIAPP transgenic islets cultured in 16.7 mmol/L glucose developed amyloid and had significantly elevated ROS levels, accompanied by increased beta cell apoptosis and reduced insulin content. NAC or Congo red treatment of hIAPP transgenic islets reduced ROS levels, amyloid deposition and beta cell apoptosis. Also, Congo red significantly increased insulin content in hIAPP transgenic islets compared to non-transgenic islets. n=4–5; *p<0.05 vs transgenic 16.7 mmol/L.