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. 2009 Aug 10;4(8):e6564. doi: 10.1371/journal.pone.0006564

Figure 3. Both multipolar prometaphase and bipolar metaphase CIN cells possess multiple centrosomes.

Figure 3

The figure shows examples of multipolar prometaphase (A) and bipolar metaphase (B) CIN cells immunostained for α-tubulin, kinetochores, and γ-tubulin. Images shown were obtained by merging maximum intensity projections of either α-tubulin and CREST (kinetochores) images (left column) or α-tubulin and γ-tubulin images (right column). A. Most multipolar prometaphase cells (exact percentages shown at the bottom right corner of the right panels) exhibited γ-tubulin staining at all spindle poles. B. Examples of bipolar metaphase CIN cells with multiple centrosomes at a single spindle pole (arrows point at γ-tubulin-stained dots). Scale bar, 5 µm. C. Frequencies of bipolar metaphase/early anaphase CIN cells possessing multiple centrosomes (as visualized by γ-tubulin staining) at a single spindle pole.