Fig. 6.

The crystallization conditions of the photosynthetic reaction center from Blastochloris viridis was screened against two sets of 24 precipitants in duplicate on the SlipChip. (a) A microphotograph of the SlipChip containing 48 crystallization trials after incubation for two hours. This image corresponds to the SlipChip after loading precipitants, adding solution of the protein, and slipping (as in Fig. 3f). Different precipitants are labeled as 1 through 24. Conditions 4 and 5 produced crystals in both sets of wells. (b) Side-by-side comparison of row 1 with row 3 and row 2 with row 4, illustrating reproducibility of the results between the duplicate sets of experiments. (c) Microphotographs of conditions 4, 5, and 6, which contained the same precipitant ((NH₄)₂SO₄) with concentrations of 3.2 M, 3.6 M, and 4.0 M. As expected, a transition from a single crystal, to multiple crystals, to heavy precipitation was observed with the increase in precipitant concentration. The scale bar is 250 µm.