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. Author manuscript; available in PMC: 2010 Jun 26.
Published in final edited form as: Mol Cell. 2009 Jun 26;34(6):641–651. doi: 10.1016/j.molcel.2009.04.034

Figure 4. Effects of Clinically-Observed JM Mutations on EGFR Activity.

Figure 4

A. The noted clinically-observed JM mutations were introduced into the Flag-tagged EGFR ICD. The resulting constructs were transiently expressed in Cos-7 cells, and ICD autophosphorylation was assessed as described in Figure 1. B. and C. The V665M, L668P, and P670L mutations were also introduced into full-length EGFR, and the influence on EGF-dependent (+) and EGF-independent (−) EGFR autophosphorylation was assessed as described for Figure 1C. The K721R inactivating mutation was included as a negative control (C).