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. Author manuscript; available in PMC: 2010 Jun 26.
Published in final edited form as: Mol Cell. 2009 Jun 26;34(6):641–651. doi: 10.1016/j.molcel.2009.04.034

Figure 6. The V665M Mutation must be present in the Acceptor Molecule to Activate EGFR.

Figure 6

ICD constructs containing mutations that limit them to functioning only as a donor (I682Q) or acceptor (V924R) in the asymmetric TKD dimer were used to assess the mechanism by which the V665M mutation activates EGFR. The noted combinations of ICD constructs were coexpressed in 293 cells as indicated, and lysates were subjected to SDS-PAGE and immunoblotting for phospho-EGFR (with anti-EGFR-pY1173) and for protein levels with anti-Myc, and anti-Flag as appropriate. Autophosphorylation levels are greater than wild-type (lane 2) only when the V665M mutation is present in the acceptor TKD (lanes 9 and 10).