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. Author manuscript; available in PMC: 2009 Aug 3.
Published in final edited form as: Nature. 2008 Jul 23;454(7207):1009–1013. doi: 10.1038/nature07160

Figure 3. EspFU induces actin pedestal formation.

Figure 3

A. Cells expressing the TirΔC, TirΔC-R2 or TirΔC-R2 VL* (with N-terminal HA-tags) were challenged with E. coli expressing intimin, and stained with anti-HA antibody (green) and Alexa568-phalloidin (red, left panels) or anti-N-WASP antibody (red, right panels). Colocalization is yellow in merged images. The % of transfected cells harboring at least five F-actin foci was quantified (Actin Assembly Index). Bacteria were visualized by DAPI staining. B. Cells expressing Tir-repeat proteins were challenged with anti-Tir antibody and S. aureus particles and Actin Assembly Index was determined. In A and B data represent mean +/− SD from at least two samples with 20-50 cells examined per sample.