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. 2009 Aug 14;5(8):e1000607. doi: 10.1371/journal.pgen.1000607

Figure 7. Immunohistochemical analysis of littermate WT (+/+) and Clrn1 KO (−/−) mouse retinas.

Figure 7

(A–D) WT and KO retinas stained with an antibody to Calbindin, shown at low (A and B) and high (C and D) magnifications. (E–H) WT and KO retinas stained with an anti-PKC-alpha antibody at low (E–F) and high (G–H) magnifications. (I,J) WT and KO retinas labeled with the rhodamine-conjugated peanut agglutinin (PNA) lectin. (K,L) WT and KO retinal sections immunostained with anti-Synaptophysin antibodies, shown at high magnification. (M–P) WT and KO retinal sections stained with intermediate filament antibodies to GFAP (M,N) and vimentin (O,P). (Q,R) Immunostaining with a candidate antibody made to CLRN1 (the Clrn1 gene product) in WT (Q) and KO (R) retinas. (S,T) Control images showing labeling by the secondary antibodies alone. Scale bar = 20 µm in all images. Scale bars: In A applies to A–B, E–F, I–J, M–N, and Q–T; in C applies to C–D, G–H, and K–L; in O, applies to O–P. INL = inner nuclear layer, ONL = outer nuclear layer. SYTOX Green was used as the nuclear counterstain.