Figure 9. Block of cholangiocyte lysis by anti-Nkg2d antibodies.

Mean (±SD) percentage of ⁵¹Cr release by the murine cholangiocyte line mCL after 5 and 24 hours of coculture with hepatic NK cells purified 7 days after injection of RRV into 1-day-old mice. Hepatic NK cells were added directly to the culture or preincubated with 50 or 100 μg of blocking anti-Nkg2d antibodies before being added to the culture. The horizontal axis shows mCL (target) to NK (effector) cell ratios. n = 3 wells per group; results are representative of 2 independent experiments, with hepatic NK cells obtained from pools of 15–20 livers (*P < 0.01).