Figure 10. Per1 mediates regulation of αENaC expression in the outer medulla.

(A) The induction of αENaC by aldosterone is attenuated in OMCD₁ cells after Per1 knockdown. OMCD₁ cells were grown, siRNA-transfected, and treated with vehicle or aldosterone as described for mIMCD-3 cells. *P < 0.05 versus non-target siRNA–transfected cells treated with vehicle; **P < 0.05 versus non-target siRNA–transfected cells treated with aldosterone; n = 4. (B) In the absence of aldosterone, Per1 knockdown results in a 2-fold decrease in αENaC mRNA levels in OMCD₁ cells. ^†P < 0.05 versus non-target siRNA–infected cells; n = 6. (C) Per1-deficient mice exhibit reduced αENaC expression in the outer medulla. ^‡P < 0.05 versus wild-type; n = 3. All data are presented as mean ± SEM.