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. 2009 Jul 1;119(8):2423–2434. doi: 10.1172/JCI36908

Figure 2. Per1 is upregulated by aldosterone in mIMCD-3 cells.

Figure 2

(A) QPCR was used to calculate changes in gene expression of Per1, Per2, Sgk1, and αENaC in aldosterone-treated compared with vehicle-treated cells. Fold change values were normalized against actin levels, relative to the vehicle control, and are presented as mean ± SD; n = 3. *P < 0.05 versus vehicle control. (B) Total cell lysate was collected from mIMCD-3 cells treated with vehicle or aldosterone for 6 hours. Western blot analysis was performed using an anti-Per1 antibody. GAPDH was used as a loading control.