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. 2009 Jul 1;119(8):2423–2434. doi: 10.1172/JCI36908

Figure 3. Per1 is transcriptionally regulated by aldosterone.

Figure 3

(A) Mouse IMCD-3 cells were transfected with pRL Renilla luciferase and either the pGL3 empty vector or a plasmid containing 2,002 bp of the Per1 promoter cloned in front of the firefly luciferase cDNA (Per1/luc). Twenty-four hours later, cells were treated with vehicle or aldosterone for 6 hours. Data are presented as mean ± SEM; n = 4. *P < 0.05 versus Per/luc plus vehicle. (B) Primers were designed to amplify a 191-bp region of the Per1 gene between exon 7 and intron 7 in order to measure hnRNA as an indicator of transcriptional activity in 3 (nos. 1–3) independent sets of template from vehicle- or aldosterone-treated mIMCD-3 cells; n = 3. L, ladder; V, vehicle; A, aldosterone. (C) An 874-bp GAPDH product was amplified as a PCR control.