Figure 8. αENaC expression is inhibited by Per1 knockdown in the absence of aldosterone.

Mouse IMCD-3 cells were transfected with a non-target siRNA or Per1-8 siRNA. Forty-eight hours later, total RNA was isolated and processed for QPCR. Fold changes in Sgk1 and αENaC were normalized against actin relative to the non-target siRNA–transfected cells. Data are presented as mean ± SEM; n = 4. *P < 0.05 versus non-target siRNA–transfected cells.