Major phenotypes of lim-7(tm674) include Unc, Pun, Dpy, constipation, defective head morphology, and increased body vacuolarization. All images are of L1 larvae visualized using DIC optics. (A) Unc, Pun and constipation phenotypes. (B) Head bump, normal pharyngeal attachment, and increased body vacuoles. (C) Dpy, Pun and constipation. (D) Rare gross head malformation. Inset is magnification of head for better detail. Arrowheads in A, B, C, end of pharynx; Arrows in A, C, distended intestine; * in B, body vacuoles. Scale bars, 20 μm.