Fig. 4.

(A) Expression of endothelial VCAM-1 (arrows) in the murine carotid artery 5 hours after an intraperitoneal injection of saline (control) or TNF-α (10 µg/kg) in the presence or absence of continuous intra-arterial infusion of [11,12]-EET or [14,15]-EET (both at 100 ng/kg per minute for 5 hours) (25). Bar, 250 µm. Effect of EETs on TNF-α–induced U937 mononuclear cell (B) adhesion and (C) rolling in the murine carotid artery. The number of adherent and rolling cells per mm² of endothelium was measured after infusion of U937-labeled cells (20). Mononuclear cell adhesion or rolling was not detected in control mice. Mice were treated with TNF-α alone (□) and in the presence of [11,12]-EET (○), [14,15]-EET (∆), or a mAb (MK2) directed against VCAM-1 (■) (23). For mononuclear cell adhesion, threeseparate experiments yielded similar results with less than 10% variation (*P < 0.05 versus TNF-α alone).