Skip to main content
. Author manuscript; available in PMC: 2009 Aug 3.
Published in final edited form as: J Biol Chem. 2005 Jan 4;280(9):7493–7503. doi: 10.1074/jbc.M411514200

FIG. 6. Interaction between SMRT receptor interaction domains and RXRα/TR heterodimers or RARα homodimers.

FIG. 6

Varying amounts of purified GST-SMRTα(S1/S2) or GST-SMRTτ(S1/S2) were added to binding reactions containing TRα1 and RXRα (A) or TRβ1 and RXRα (B) and a DR4 oligonucleotide probe. Alternatively, varying amounts of purified GST-SMRTα(S1) or GSTSMRT α(S1) (C), GST-SMRT(S2) (D), or GST-SMRTα(S1/S2) or GST-SMRTτ(S1/S2) (E) were added to binding reactions containing RARα and a radiolabeled DR5 oligonucleotide probe. The receptor·DNA complexes supershifted by addition of the SMRT constructs were quantified relative to the amount of SMRT protein added to each binding reaction. Error bars indicate S.E. of three replicate experiments. RID, receptor interaction domain.