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. Author manuscript; available in PMC: 2009 Aug 3.
Published in final edited form as: J Biol Chem. 2005 Jan 4;280(9):7493–7503. doi: 10.1074/jbc.M411514200

FIG. 7. Disruption of SMRT·TR complexes with thyroid hormone.

FIG. 7

A fixed amount of purified GST-SMRTα(S1) or GST-SMRTτ(S1) were added to binding reactions containing TRβ1 (A) or TRα1 (C) together with a radiolabeled DR4 oligonucleotide probe. Alternatively, a fixed amount of purified GST-SMRTα(S1/S2) or GST-SMRTτ(S1/S2) were added to binding reactions containing TRβ1 (B) or TRα1 (D) together with the radiolabeled DR4 probe. Varying amounts of T3 were added to each reaction. The SMRT-TR-DNA complexes formed in the presence of the differing T3 concentrations were quantified. (SMRT-TR-DNA complexes observed in the absence of hormone were defined as 100%.) Error bars for each data point indicate S.E. (n = 3).